Effect of β-glucan on the immune response of early stage of Anabas testudineus (Bloch) challenged with fungus Saprolegnia parasitica
نویسندگان
چکیده
The present study was carried out to study the effect of different dosages of β-glucan suspension on immune response and disease resistance in Anabas testudineus spawns against a fungal pathogen Saprolegnia parasitica. Eight day old spawns were exposed for 3 h in four different dosages of β-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l(-1). The cell suspension of spawn was assayed for total protein, acid phosphate, lysozyme, bactericidal and NBT activity. The spawns were then challenged with 3x10(5) CFU ml(-1) of S. parasitica. The survivability percentage and immunological parameters were assayed upto day 7. Exposure of fish for 3h to all the concentrations of β-glucan recorded a significant enhancement in the immunological parameters such as lysozyme activity, bactericidal activity and NBT activity by the end of day 7. The challenge study indicated least mortality in the groups exposed to 10 mgl(-1) and 15 mgl(-1) but 15 mgl(-1) gives little higher survivability. Thus 3 h exposure of β-glucan suspension could reduce the mortality and increase the immunity of A. testudineus spawns against S. parasitica.
منابع مشابه
Effect of curcumin supplementation on hepatic, renal and intestinal organization of Anabas testudineus (Bloch): Light and electron microscopic studies
Anabas testudineus (commonly known as ‘climbing perch’) is a euryhaline teleost, inhabiting both freshwater and brackish water ecosystems of India and other South East Asian countries. It thrives well in water deficient in dissolved oxygen and is capable of migrating between ponds taking advantage of the accessory respiratory organs, the labyrinthine organs. The market demand for this fish is v...
متن کاملClimbing Perch Anabas Testudineus Bloch I. Oxygen Uptake and Carbon Dioxide Release into Air and Water
متن کامل
NOTES Rapid regulatory effect of tri-iodothyronine (T3) on antioxidant enzyme activities in a fish Anabas testudineus (Bloch): Short-term in vivo and in vitro study
The short-term action of thyroid hormone tri-iodothyronine (T3) was studied in vivo and in vitro on antioxidant enzyme activities in a teleost Anabas testudineus (Bloch). T3 injection in vivo (200 ng) in normal fish decreased the lipid peroxidation products and increased superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities after 30 min. T3 in vitro (10 M) increased t...
متن کاملIrradiated β-glucan enhances immune response to bacterial infection through CD4 and CD8 T-lymphocytes
Background: &beta-glucans are glucose polymers with a variety of stimulatory effects on the immune system. The objective of this study was to evaluate the immune-enhancing activities of low molecular weight gamma irradiated &beta-glucan (I&beta-g) extracted from Pleurotus ostreatus in response to Pseudomonas aeruginosa infection in rats. Materials and Methods: &beta-glucan (&beta-g) powder was ...
متن کاملThree New Species of Myxobolus Bütschli, 1882 from Different Food Fishes of West Bengal, India
Investigations on the incidence of myxozoan parasites (Myxozoa: Bivalvulida) in fishes have assumed immense importance in view of severe pathogenicity and mortality caused by these organisms on their hosts. The present communication records three new species of myxosporeans, Myxobolus calcariferum sp. n.; Myxobolus chinsurahensis sp. n. and Myxobolus mrigalhitae sp. n. from different food fishe...
متن کامل